Description
Principle of the assay: The microtiter plate provided in this kit has been pre-coated with anti-mouse IgG antibody. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antigen and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of anti-nuclear antibody(IgG) in the samples is then determined by comparing the O.D. of the samples to the standard curve